In-vitro maturation of mouse oocytes
نویسندگان
چکیده
منابع مشابه
P-87: High Fat Diet Affects In Vitro Maturation and In Vitro Fertilization of Mouse Oocytes
Background: Physiological effects of obesity are increasingly common in women of reproductive age and are associated with infertility. Obese women have dysfunction in hypothalamic-pituitary-ovarian axis, menstrual cycle and are up to three times more likely to have decreased oligo-/anovulation. The purpose of present study was to evaluate the high fat diet on IVM and IVF outcomes in mouse. Mate...
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هدف از آزمایش اول بررسی اثر سطوح مختلف سریسین [0 (control), 0.1, 0.5, 1.0, 2.5 %] افزوده شده به محیط , ivm بر cumulus cell expansion، بلوغ هسته و توسعه متوالی جنین، در گوسفندان نژاد سنجابی در فصل تولید مثلی می باشد. از سرگیری میوز به وسیله خارج شدن اولین پولار بادی اندازه گیری و هم چنین درصد رسیدن جنین های دو سلولی به مرحله کلیواژ و بلاستوسیت نیز به عنوان نشانه ای از میزان شایستگی توسعه اولیه ج...
The Effect of Retinoic Acid on in Vitro Maturation of Mouse Immature Oocytes: An Experimental Study
Background and aims: Various culture media along with some supplements and enrichment materials have been suggested for in vitro maturation. This study aims to investigate the effect of retinoic acid on nuclear maturation of immature mouse oocytes in vitro. Materials and methods: immature oocytes were obtained from 6-8 weeks old NMRI mice, 48 hours after intraperitoneal injection of 7.5 units ...
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Purpose: This Study evaluated the resumption of meiosis, in vitro maturtion and department of immature mouse oocytes with b- mercaptothanol. Materials and methods: Immature oocytes were recovered from NMRI mouse strain (4-6 weeks) in different 4 experiments groups: the control group, the oocytes were matured in MEM-a+ medium containing + FCS 55%; group 1: the oocytes were matured in MEM- a med...
متن کاملThe Influence of Meiotic Spindle Configuration by Cysteamine during in vitro Maturation of Mouse Oocytes
Background: The aim of this study was to assess effects of cysteamine as an anti-oxidant on rate of in vitro maturation of oocyte and determination of its effects on spindle size and shape. Methods: Pre-mature mice were primed with pregnant mare stimulating gonadotrophin (PMSG) and germinal vesicle (GV) stage oocytes were obtained 48 h after. The oocytes were cultured in tissue culture medium (...
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ژورنال
عنوان ژورنال: Human Reproduction
سال: 1996
ISSN: 0268-1161,1460-2350
DOI: 10.1093/oxfordjournals.humrep.a019105